Useful Links & Tools
- MSDS
- Certificate of Analysis
- Certificate of Origin
- F3422 - Datasheet (84KB)
- Bulk Quote
- Similar Products
Last 5 Products Viewed
F3422
Sigma
Flt-3/Flk-2 ligand human
≥98% (SDS-PAGE and HPLC), recombinant, expressed in Escherichia coli, lyophilized powder, suitable for cell culture
| Synonym: | FL |
| MDL number: | MFCD01093789 |
Description
| Analysis Note | The biological activity is measured by its ability to stimulate the proliferation of human AML5 cells. |
| General description | Mouse and human Flt-3 ligand share ~72% homology and both demonstrate cross-species activity. |
| Physical form | Lyophilized, 0.2 μm sterile filtered, with with 0.5x PBS. |
| Biochem/physiol Actions | Flt-3/Flk-2 Ligand (FL, or Flt-3L) is a transmembrane glycoprotein that binds and activates the Flt-3 receptor. Expression of Flt-3 is primarily restricted among hematopoietic cells to the most primitive progenitor cells. |
Properties
| recombinant | expressed in Escherichia coli |
| assay | ≥98% (SDS-PAGE and HPLC) |
| form | lyophilized powder |
| total impurities | Endotoxin, tested |
| suitability | suitable for cell culture |
| Gene Information | human ... FLT3(2322) |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Lyman, S., et al., Thomson, A.W. The Cytokine Handbook 3rd ed., San Diego, CA , (1998), 727 |
Namikawa, R., et al., Regulatory roles of the ligand for Flt-3/Fc tyrosine kinase receptor on human hematopoiesis. Stem Cells 14, 388-395, (1996) Abstract | |
| Rosnet, O., et al., Murine Flt3, a gene encoding a novel tyrosine kinase receptor of the PDGFR/CSF1R family. Oncogene 6, 1641-1650, (1991) Abstract | |
| Matthews, W., et al., A receptor tyrosine kinase specific to hematopoietic stem and progenitor cell-enriched populations. Cell 65, 1143, (1991) Abstract | |
| Lyman, S., et al., Characterization of the protein encoded by the flt3 (flk2) receptor-like tyrosine kinase gene. Oncogene 8, 815-822, (1993) Abstract | |
| Hannum, C., et al., Ligand for FLT3/FLK2 receptor tyrosine kinase regulates growth of haematopoietic stem cells and is encoded by variant RNAs. Nature 368, 643-648, (1994) Abstract | |
| Drexler, H., et al., Effects of FLT3 ligand on proliferation and survival of myeloid leukemia cells. Leukemia Lymphoma 33, 83, (1999) Abstract |
